Biocompatibility of Intravenous Nano Hydroxyapatite in Male Rats

This study was carried out to evaluate the potential impact of nano hydroxyapatite (nano-HAp) intravenous injection with different concentrations on survival rates and the reaction affecting heart, liver and thyroid functions as well as the level of circulating nitric oxide. An inorganic-organic complexion route was selected for the synthesis of nano-HAp which was characterized by fourier transform infrared, x-ray diffraction, transmission electron microscope, and analyzed using inductively coupled plasma mass spectrometry. The synthesized nano HAp was thermally sterilized and prepared with different concentrations (150, 300, 600, 1200, 1800 and 2400 mg/kg b.w.) to be injected intravenously into different groups of male rats. Mortality percentage was recorded throughout the experiment. Blood samples were collected at time intervals of 1⁄2, 3, 6, 12, 24 and 48 hours after injection. The present results revealed that intravenous injection of nanoHAp at a dose of 2400 mg/kg b.w. was determined as lethal dose, where all the animals in this group died within ten minutes after injection. On the other hand, the amount of 150 mg/kg b.w. nano-HAp did not affect on the investigated parameters but the amount of 300 mg/kg b.w. appeared slightly variable changes in CKMB, LDH, AST and T3 recovered after 24 hours. The animals injected with 600, 120